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• 7725I英文說明書

  7725I英文說明書[詳細]

  2024-09-19 04:04

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• 7725I中文說明書

  7725I中文說明書[詳細]

  2011-04-11 00:00

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• 7725i進樣閥中文使用說明書

  本說明書適應于型號7725i，9725i，3725i-038，3725i，和9125手動進樣器。型號7725i，9725i，3725i-038，3725i，和9125是樣品通過閥前面的內嵌式針道被載入的六通閥。型號7725i和3725i-038是不銹鋼的，9125是PEEK制成的。型號3725i-038和3725i是接收1/8``量的準備階段的進樣器。除了進行準備階段的應用之外，準備階段的進樣器和它們相應的分析階段的進樣器是一樣的。[詳細]

  2024-09-28 01:08

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• （離心機）英文說明書

  BriefintroductionandapplicationscopeAscentrifugalseparator,thiselectriccentrifugalmachinemakesuseofthecentrifugalforcetoseparateeverycompositioninsolution,adoptsteplessspeedvariationandautomaticbalancedevices,sothismachineownsvariousadvantages,suchasstableperformance,smallvolume,beautifulappearance,lowertemperaturerising,highworkingefficiencyandwiderapplication,speciallyforitsaluminumalloycentrifugalrotary-disc,itcanguaranteetoprotectthecentrifugaltubesfromdamageeffectively.Thus,thiscentrifugalseparatorhasbeenwidelyusedinhospitalandchemicalorbiologylabtocarryoutqualitativeanalysisforbloodserum,bloodplasma,etc.Technicalparameters:Model800800D80-2TDL-50XYJ-3XYJ-4TDL-5Max.rev4000rpm5000rpmRCFMac1430（xg）1790（xg）5000（xg）Max.capacity20mlx6（Angietype）20mlx1220mlx810mlx16；50mlx8100mlx4Motorpower25W40W280WTimingrangeNo0-60min0-120minAutobalanceNoYesYesSelf-lockerNoNoNoNoNoYesWeight67121035ImportantNotice：●Foryoursafety,areliablegroundingisstronglyrecommendedtobedonebeforeoperation,●Beforethefirsttimeuse,pleasereadthisOperationManualcarefully.●Workingconditions:0-40℃ambienttemperatureand80%belowrelativehumidity.●Itissuggestedtocutoffpowerwhenlongtermnonuse.OperationProcedure:●Inserttheplugintosocketandturnonthepowerswitch.●Putthemedicineintocentrifugetubeandtheninsertthetubeintothecorrespondingsleeve,closethecoverplateandselectpropercentrifugalspeedaccordingtotestrequirements,thenselectrunningtimeorcloseON.Soitiseasytooperatethisinstrumentwithbettereffect.Dailymaintenance1.Correctoperationandmaintenancecanextendtheservicelife,itissuggestedtoplacethisinstrumentunderreasonableworkingconditionssothattheinstrumentcanworknormally.2.Iftheliquidissplashedorsprayedintoinstrumentbyaccident,cutoffthepowersupplyandremovetheresidualliquidincaseshortentheservicelife.Oneyearwarrantyandlifelongmaintenanceareofferedtoourproduct（reasonablelaborandmaterialcostwillbetriggeredbyman-madedamageormisoperation）[詳細]

  2018-08-21 10:00

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• 2406N英文說明書

  由深圳市朗普電子科技有限公司代理深圳市深南中路南光捷佳大廈1402室TEL:0755-88851600（直線）0755-839801580755-830474150755-83986300FAX:0755-888505150755-83047419網址：綜合推廣網www.17lp.com衡器烘箱網www.1718sz.com郵箱：lp@df17.comQQ:　374542908MSN：dafusz@hotmail.com[詳細]

  2018-09-19 10:01

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• 7725i進樣器維修小方法

  7725i進樣器維修小方法[詳細]

  2007-09-21 00:00

  安裝說明

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• SLPe使用說明書（英文）

  SLPe使用說明書（英文）[詳細]

  2012-07-09 00:00

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• XK3190-A12E儀表英文說明書

  XK3190-A12E儀表校正操作方法如下：1、在校正電子地磅時應清空秤體臺面2、要檢查電子地磅的四角角差是否在允許范圍內。3、打開XK3190-A12E稱重顯示儀表，開機過程中按住井號鍵不放，顯示一串9后放手4、按一次功能鍵，進入零位校正，如秤臺上無物品，四角差在范圍內則按一次井號鍵進入砝碼校正。5、放上砝碼（大于1/3滿量程），通過去皮鍵（用于移動位置）與置零鍵（0－9數字鍵）輸入砝碼值后稍等10幾秒后按井號鍵顯示END再按一次儀表后面的標定開關一次，校正完畢，再重新放一個砝碼測試一下，如有數據有差別，那就要重新要檢查一下電子地磅是否還存角差問題。再另重新標定校正一次。如稱重數據等同砝碼重量，則客戶即可使用。具體操作方法示意圖：[詳細]

  2018-09-26 10:00

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• 孔雀石綠英文說明書.pdf

  malachitegreen（MG）ELISA1UsepurposeThiskitforFeed,aquaticsamples,watersamplesintheMGremainingquantitativedetection.2ExperimentalprincipleThiskitadoptsmethodsincompetitionELISAInmicroplatescoatedwithantigenconjugatedMG,addMGstandardorsamples,FreeMGandpre-coatedonstripsofMGconjugatedantigencompeteagainstMGantibodyconjugates,WithTMBchromogenicsubstrate,thecolorischangedfrombluetoyellowafteraddingstopsolution,enzymestandardinstrument450nmwavelengthsintesting,absorblightvalueandinthesamplewasMGcontentisinverselyproportionaltothestandardcurve,throughcalculationsampleswasMGconcentrations.3Materialsprovidedwiththekit3.1Microelisastripplate:1block（12well×8strips）.3.2MGstandard:sixvialsof（1ml/vial）,contentisrespectively:0PPB,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB.3.3Anti-MGantibodyconjugate:1vial（6ml）.3.4ChromogenSolutionA:1vial（6ml）.3.5ChromogenSolutionB:1vial（6ml）.3.6StopSolution:1vial（6ml）,2Msulphuricacid.3.7sampledilution:1vial（10x,6ml）,usedforsampledilutedwith.3.8washsolution:1vial（20x,20ml）,usedforwashingboard.3.9Instruction.4Neednotprovidematerials4.1equipment4.4.1wavelength450nmmicroplate.4.1.2shredder.4.1.3LiangTong.4.1.4oscillators.4.1.5funnel.4.1.6WhatmanNo1orequivalentfilterpaper.4.1.7traceremoveliquiddevice.4.2reagent4.2.1thedeionizedwaterordistilledwater.4.2.2methanol.5Storage5.1kitstoredin2~8℃,Don’tfrozen5.2Don’tuseuptheMicroelisastripplate首ldbesealeddryingpreserve6.Precautions6.1pleasereadtheinstructionscarefully,beforeuseingthekit.6.2don'tuseexpiredkit.6.3beforeusingthekit,pleaseletthereagentrecovertoroomtemperature（25+2℃）,theproposalforatleast2hourstotemperature.6.4thestandardcontainMG,payspecialattentionto,theoperation,we首ldbringgloves.6.5stopsolutioniscontainingsulfate,whenusing,preventburnsskinandcorrosionclothing.6.6differentstandardandsamplesuctionheadusedcannotbemixeduse,otherwise,itwillaffecttestresults.6.7differentbatchesofreagentkitnotmix,Differentstandardandsamplesuctionheadshallnotbeusedincombination,otherwise,itwillaffecttheexperimentalresult.6.8dilutedsamplemustusethiskitofsamplediluent,otherwise,itwillaffecttheexperimentalresult6.9mixedreagents首ldavoidblistering.7Workingliquidpreparation7.1Carbendazimstandards:0ppb,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB7.2washsolution:1:20withdistilledwater（1+19）diluted.preparation7.3sampledilutions:1:10withdistilledwater（1+9）diluted.preparation7.3ChromogenSolutionreagent:alreadystandby,avoidlightstraightas7.4stopsolution:alreadyterminationaside8Sampleprocessingprogram（sampleinextractionprocess,muststrictlyaccordingtotheoperationoftheextractionprocess首ldbeaccuratedilution,canappearotherwiseresultsareinaccurate,samples首ldbekeptinacoolplacetoavoidlightandfrozenkeep）8.1Smashthesamplestaken10g,add20ml70%methanolsolution8.2powerfuloscillation3minutes8.3WhatmanNo1filterwith8.4Takethe25μltreatmentofthesamplebyadding25μlsampledilutioninthewells（sampledilutionfactorof2）9Enzyme2lindedanalysissteps9.1experimentalguidelines9.1.1experimentbeginspriortoallreagentinboxesoutsidetheroomtemperature（25fullyrecoveredto+2℃）,timeabout2hours.Returntoroomtemperature（25+2℃）againafterremoveStrips,excessporebartosealimmediatelytothe2~8℃dryingpreserveNote:besuretotemperature,otherwisefullyguaranteetheaccuracyandprecisionoftheaffectingdetection.9.1.2afterusepleaseimmediatelyreagentputback2~8℃preservation9.1.3pleasedon'tchangeanalysisprogram9.1.4pleaseuseaccuratetraceremoveliquiddevice9.1.5operationoncestarted,pleasedonotinterruptanyprogram9.1.6ELISAresultsofrepeatabilityofseveredependsonoperatingprocedures,pleasestrictlyaccordingtorequirementsoperation9.1.7toavoidcross-contamination,eachstandardandsamples首ldusedifferentsuctionheadaddsamples9.1.8plussampledomakesuckaheadcontactmicroporousthesolutionorinsidesurface9.2analysissteps9.2.1beforehandnumbered,markB0,standardandthesamplepositionrecommendeddoubleorificedetection9.2.2taketherequiredamountoftheStrips（Stripsdetachable）,willspareattribwattleandsealedimmediatelyputbackagain2~8℃preservation9.2.3sampledilutions（10）,washsolutionx（20x）dilutionintoworkingliquid（distilledwaterordeionizedwaterdilute）In9.2.4B0welljoining50μl0.0ng/mlstandard9.2.5ineachstandardwelljoining50μlstandard9.2.6ineachsamplewelljoining50μlsamplesolution9.2.7Inallwelljoining50μlAnti-MGantibodyconjugate9.2.8gentlysloshingresponseboardforafewseconds.9.337℃warmbath30min（warmbathprocesssometimespatreactionplate,canreducedoubleorificeerror）9.3.1UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat..9.4reaction9.4.1washingprocedurecompleted,immediatelywithliquidapparatusineverytracemovemicroporousfirstjoin50μlChromogenSolutionA,add50μlChromogenSolutionB,Slightsloshingresponseboardmakethoroughlyincorporated379.4.2℃warmbath10min9.4.3eachwelljoining50μlStopSolution,blending9.4.4in450nmtestingabsorbency,resultin5mininsideread.10Theresultscalculated10.1quantitativeanalysis10.1.1obtainedbyeachconcentrationstandardsolutionandtheaveragevalueofasamplespectrophotometry（B）dividedbythefirststandard（0standardabsorbencyvalue（B0）multiplyingby1**%,namelypercentageabsorbencyvalues.B-standardsolutionorsamplesolutionofaverageabsorbanceofthevaluesB0-0μg/Lstandardsolutionofaverageabsorbanceofthevalues10.1.2withMGconcentrationsofvaluesfortheXaxis,100centabsorbanceofthevalueoftheYaxis,drawstandardcurve.Accordingtothesamplepercentageabsorbencyvalues,whichgetcorrespondingpointsfromcurve,namelytheabscissadenotesthemulti-goalMGconcentrationonthenumericalcurvature.theagainstseveralnamelytodetermineMGconcentrationC（ppb）10.1.3becausethesampleafterdilutedinadvance,soaccordingtothestandardcurvegainsfromdifferentconcentrationsamplesmustagainmultiplythedilutedtimes.10.2halfquantitatively10.1.1visualhalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesamplesandstandardsubstanceabsorbencyvaluethediscretionofthejudgeiscompared,samplechromavalueislessthanorgreaterthanstandardvalues.10.1.2instrumenthalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesampleandstandardcolordepthcomparison,judgesamplechromavalueislessthanorgreaterthanstandardvalues.11SpecificityPhysicalcrossreactionMG1**%CrystalViolet:95%Recessivemalachitegreen:0.1%HiddenCrystalViolet:<0.1%12KitparametersThiskitdetectionlimitis0.05PPBB0absorbanceoftheoptimalvalue首ldbegreaterthan1.0Kitabsorbencyboardinsideerrorislessthan8%,boardbetweenerrorislessthan15%.Withthismanualprovidedatissuesampleextractionmethodrecoveryisgreaterthan80%.13AnalysisrestrictionThiskittestingpositiveforsamples首lduseanothermethodsuchasHPLCorGC/MStobeverified.上海恒遠生物科技有限公司，專業銷售“孔雀石綠試劑盒”品質保證，值得信賴！如果你想了解該產品的詳細信息，歡迎來電來函！[詳細]

  2018-11-15 10:03

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• 電解測厚儀英文說明書

  主要針對一些院校外國留學生，對中文還不太懂的學生和出售到國外而準備的。[詳細]

  2018-12-10 15:26

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• FP640火焰光度計英文使用說明書

  FP640火焰光度計英文使用說明書[詳細]

  2014-08-08 00:00

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• FP6410火焰光度計英文使用說明書

  FP6410火焰光度計英文使用說明書[詳細]

  2014-08-08 00:00

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• 無線吊秤C表英文說明書

  線數傳式吊秤C型儀表產品特點:1,內置EPSON打印機,可以打印完整的稱重數據.2,多達2900組稱重數據存儲,支持中文題頭輸入。3，完整的互換性，模塊化的設計，維修變得更簡單。4，全開放的程序，根據不同場合查以調整和設定5，可選配RS232串行輸出，實現與上位機通訊。6，可選配4-20mA遠程傳輸模塊。無線數傳式吊秤C型儀表[詳細]

  2018-09-30 10:01

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• XK3190-C8控制儀表英文說明書

  XK3190-C8控制儀表英文說明書XK3190-C8儀表采用高速的Σ-△模數轉換技術。適用于電子配料秤、定值秤、分選秤等控制場合，同時具有上下限報警、峰值保持功能。3路光隔離開關量輸入，3路開關量（內置3路繼電器）輸出控制高精度A/D轉換，可讀性達1/300005點非線性修正換表無需重新標定，只需輸入原儀表的參數即可使用集加/減法秤/分選秤/上下限報警/峰值保持等功能于一體定量值、提前量、上下限、節點延時等參數可根據應用自由設置加/減法秤模式下可選1-2種配料，分選秤模式下可選外控/自檢/自由上下限模式準確度等級：3級，nind=3000AD轉換方式：Σ-△轉換方式AD轉換碼：24bit轉換速度：80次/秒輸入信號范圍：-19mV～+19mV輸入靈敏度：≥1uV/e非線性：≤0.01%F.S滿量程溫度系數：≤8PPM/℃傳感器供橋電源：DC：5V；350mA傳感器連接個數：1~8[詳細]

  2018-10-07 10:04

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• HC316壓力變送器英文使用說明書

  HC316系列壓力變送器采用進口擴散硅或陶瓷芯體作為壓力檢測元件，傳感器信號經高性能電子放大器轉換成0~ 10mA或4~ 20mA統一輸出信號。可替代傳統的遠傳壓力表、霍爾元件、差動變送器。能與各種型號的動圈式指示儀、數字壓力表、電子電位差計配套使用，也能與各種自動調節系統或計算機系統配套使用。廣泛用于電力、石化、機械、冶金等行業及各種能源計量領域[詳細]

  2020-10-16 09:41

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• DJH MEK摩擦試驗機英文說明書

  DJHMEK摩擦試驗機英文說明書[詳細]

  2018-08-16 10:00

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• 便攜式地磅（汽車衡）英文說明書

  概述行駛道路的車輛超限超載是造成道路加速損壞以及引發交通事故的潛在重要原因，隨著《道路交通安全法》的實施，我國交通和JJ等相關部門對超限超載的執法力度不斷加強，同時相關執法部門更需要一種能夠在其執法過程中極ng確檢測車輛動態重量的高質量設備。為了給相關執法管理部門提供一個便捷、GX的執法工具，提供有力的執法依據，根據交通部及有關部委對車輛軸類型和軸載質量的相關規定標準，我公司于1999年初自主研發生產出符合我國車輛行駛公路管理規范要求的便攜式稱重儀（軸重儀），為交通管理部門實施有效的超限超載治理提供了強有力的執法工具。您所使用的便攜式稱重儀是我公司基于自身多年的實際動態稱重經驗以及客戶的實際需要，而Zxin研發生產的JJ系列新一代全數字便攜式稱重儀（軸重儀），旨在改善提高目前市場上同類產品不能滿足用戶的實際使用需求而研發生產，具有多項創新功能，主要體現在：1、采用7寸全彩觸摸式大屏顯示，完全滿足用戶在夜間操作的方便性2、國內SJ將電腦技術應用于便攜式動態稱重儀領域，即采用用戶熟悉的鼠標操作方式，使得產品實用性更強，操作更簡單明了，大大提高操作人員的工作效率。3、國內SJ推出JJ和路政聯合執法模式功能4、國內SJ推出功能性更強的后臺軟件管理功能，只要把我們的儀表與安裝有后臺管理軟件的計算機連接，就可以實時的控制儀表操作，為更高功能的擴展打下基礎產品設計定位高、精度高、性能穩定、使用可靠、環保節能、故障率低、維修簡捷等特點。可方便的對機動車進行軸載質量、車貨總質量的動、靜態測量。適用于公路執法部門超限檢查、JJ進行車載質量檢查、高速公路收費站進行車載質量計量等，也適用于低值車載貨物計量，如交通運輸車載質量計量、建筑施工工地配料計量等場合。一、主要功能特點:（1）、傳感器采用一體化整體式結構整體式傳感器（2）、數據傳輸方式：有線式、無線式、有線無線兩用式（具體傳輸方式根據用戶的實際需要在采購是選擇而定）（3）、采用7寸彩色觸摸式屏幕顯示，實用（4）、可以使用觸摸方式輸入操作和無線鼠標方式操作，簡單快捷（5）、多種工作（JJ、路政、綜合）模式可供選擇（6）、動、靜兩用、性能穩定、具有高性能防水、防振、防腐等特點（7）、雙通道式設計、使用高精度整體式傳感器，檢測精度高，故障率低（8）、統計分析軟件，便于記錄、統計、查詢；數據庫提供車型資料、政策法規、技術支持（9）、即時電腦連接操作，可以通過電腦和儀表連接直接操作，并可實現視頻和車牌自動識別功能擴展功能強大（此功能為選配）二、主要技術參數:1、名稱型號：便攜式稱重儀（JJB30BW型）2、靜態精度：靜態稱重（III）；±0.1～0.3%FS蠕變：0.3%.F.S，遲滯：0.5%.F.S3、動態精度：OIML國際建議級1%-3%F.s非線性：0.5%.F.S，重復性：0.3%.F.S4、分度值：5、10、20kg等可調節5、Zda稱重量：40000kg（單軸）6、Z小秤量：20e（e為檢定分度值）7、Zda安全載荷：安全超載能力：120%F.SZda超載能力：150%F.s8、使用環境：-20~80℃9、電源：DC12V、AC220V、車載供電三種供電方式10、引板：橡膠引板11、限制車速：≤5km/h勻速12、打印內容：牌照、車貨總質量、軸胎形式、限載值、超限值、時間等（具體打印內容依據客戶的要求而定）13、軟件：具有3種稱重軟件（路政、JJ、綜合模式）且可以自由切換14、數據輸出：RS232、USB兩種數據接口15、傳感器結構：采用整體數字傳感器，溫度補償，可以在秤臺溫度變化較大時對溫度產生的誤差進行補償，同時傳感器采用雙信號電路設計，在一個電路出現故障的同時，系統會自動切換到另外一個電路，提高現場設備的實用性，減少設備的故障率16、軸型表模式：軸型表采用內部電子顯示方式設定，徹底解決長期野外使用貼表式軸型表模糊不清以及操作不便的問題17、視頻：支持即時視頻聯接、支持車牌自動識別系統、支持后臺軟件擴展（此功能為選配）18、打印機:采用針式節能高速打印機，可保存打印內容，徹底解決熱敏打印機高耗電、而且打印內容容易自然消退的問題電子秤（上海電子秤）地磅秤（小地磅秤）大臺面地磅秤（電子磅秤）吊秤（上海吊秤）[詳細]

  2024-09-30 17:41

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• NDJ-8S數顯旋轉粘度計英文說明書

  NDJ-8S數顯旋轉粘度計英文說明書[詳細]

  2009-10-13 00:00

  報價單

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• 人L-精氨酸（L-ARG）英文說明書

  HumanL-Arginine（L-ARG）FORRESEARCHUSEONLYAssayrange：2nmol/L-48nmol/L96determinationsPurposeThiskitallowsforthedeterminationofL-ARGconcentrationsinHumanserum,cellculturesupernatesandotherbiologicalfluidsPrincipleoftheassayThekitassayHumanL-ARGlevelinthesample,usePurifiedHumanL-ARGantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddL-ARGtowells,CombinedL-ARGantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofHumanL-ARGinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（96nmol/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:48nmol/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent24nmol/L4Standard150μl5Standard+150μlStandarddiluent12nmol/L3Standard150μl4Standard+150μlStandarddiluent6nmol/L2Standard150μl3Standard+150μlStandarddiluent3nmol/L1Standard150μl2Standard+150μlStandarddiluent2.Addsample:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.Washing:UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.Addenzyme:AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.Incubate:Operationwith3.8.Washing:Operationwith5.9.Color:AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction:AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.Assay:takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor30minat37℃.AddStoppSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[詳細]

  2018-09-19 10:00

  產品樣冊

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• 豬DC細胞（DC）英文說明書

  PorcineDCFORRESEARCHUSEONLYAssayrange：1.0ng/L-60ng/L96determinationsPurposeThiskitallowsforthedeterminationofDCconcentrationsinPorcineserum,cellculturesupernatesandotherbiologicalfluidsPrincipleoftheassayThekitassayPorcineDClevelinthesample，usePurifiedPorcineDCantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddDCtowells,CombinedDCantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofPorcineDCinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（120ng/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:60ng/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent30ng/L4Standard150μl5Standard+150μlStandarddiluent15ng/L3Standard150μl4Standard+150μlStandarddiluent7.5ng/L2Standard150μl3Standard+150μlStandarddiluent3.75ng/L1Standard150μl2Standard+150μlStandarddiluent2.addsample：Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionB50ultoeachwell,evadethelightpreservationfor10minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor10minat37℃.AddStopSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[詳細]

  2018-09-19 10:00

  產品樣冊

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