描述 Thermo Scientific Pierce Protein L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein L, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

? Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
? Orient antibodies for maximum antigen-binding capacity
? Immobilize antibodies for plate assays without prior purification
? Ensure minimal variation (<5% well-to-well) from consistent coating
? Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
? Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein L Coated Microplates:
? Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
? Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
? Binds to Fab fragments and ScFv
? Does not bind bovine, goat or sheep immunoglobulin
? Binds weakly to rabbit immunoglobulin
? Pre-blocked to reduce nonspecific binding

Related Products
Pierce? Protein A Coated Plates, Clear, 8-Well Strip
Pierce? Protein A/G Coated Plates, Clear, 8-Well Strip
Pierce? Protein G Coated Plates, Clear, 8-Well Strip

For Research Use Only. Not for use in diagnostic procedures.