分子別名（Synonyms）

APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha

表達區間及表達系統（Source）

Ferret TNF alpha?Protein is expressed from E.coli with His tag at the C-Terminus. It contains Val77-Leu233.[Accession | A3FBF1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 18.88 kDa same as SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Ferret TNF-alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.