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Recombinant Human PDGF-CC Protein,His Tag
- 品牌：翌圣生物
- 產地：
- 供應商報價：面議
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翌圣生物科技(上海)股份有限公司
更新時間：2024-12-24 09:03:45 -
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詳細介紹
Specifications
Product name
Cat.No.
Size
Recombinant Human PDGF-CC Protein,His Tag
91606ES10
10 μg
91606ES60
100 μg
Description
The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD.The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules' epithelial cell development.
Product information
Synonyms
PDGFCC; PDGF-CC
Source
E.coli
Molecular Weight
Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.
AA Sequence
Accession :NP_057289 Val235-Gly345, with an N-terminal Met and 6-His tag;
Biological Activity
Measured in a cell proliferation assay using NR6R 3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/mL.
Purity
> 97% by SDS-PAGE.
Endotoxin
<0.1EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions.
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
1 month, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Notes
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230630
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