分子別名（Synonyms）

Notch-1; 9930111A19Rik; NOTCH1; Mis6

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala19-Gln526.[Accession | P46531]

分子量大小（Molecular Weight）

The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

SPR Data: Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 μM as determined in SPR assay .

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.