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Recombinant Human HPV16 E7 (HLA-A*02:01) Tetramer Protein,His-Avi Tag

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詳細介紹

性能參數

表達區間及表達系統（Source）	Recombinant Human HPV16 E7(HLA-A02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A02:01),Ile21-Met119(B2M) and YMLDLQPET peptide.[Accession \| P04439-1(HLA-A*02:01)&P61769(B2M)&YMLDLQPET peptide]
分子量大?。?/font>Molecular Weight）	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
內毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
純度（Purity）	> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC
制劑（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重構方法（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

儲存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Ver. CN20230526