表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse CD47 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Gln19-Lys140.[Accession | Q61735-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

SPR Data: Mouse CD47, hFc Tag captured on CM5 Chip via Protein A can bind Mouse SIRP alpha, His Tag with an affinity constant of 28.33 nM as determined in SPR assay 

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.