分子別名（Synonyms）

Alkaline phosphatase, placental-like;PLAP-like;ALP-1;Alkaline Phosphatase

表達區間及表達系統（Source）

Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile20-Asp503.[Accession | P10696]

分子量大小（Molecular Weight）

The protein has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Human Alkaline Phosphatase (Germ Type) , hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.