分子別名（Synonyms）

A33; GPA33;Glycoprotein A33; MGC129987

表達區間及表達系統（Source）

Biotinylated Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ile22-Val235.[Accession | Q99795-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on SDS-PAGE result.

內毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 33.2ng/ml determined by ELISA.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.