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Recombinant Cynomolgus CD3E & CD3D/CD3 epsilon & CD3 delta (hFc Tag)
- 品牌:翌圣生物
- 產地:
- 供應商報價:面議
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翌圣生物科技(上海)股份有限公司
更新時間:2024-12-24 09:03:45
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入駐年限第1年
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詳細介紹
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
產品性質
別名
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Uniprot No.
Q95LI5.2(CD3E)&Q95LI8(CD3D)
表達區間及表達系統
Recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Gln22-Asp117 (CD3E) & Phe22 - Ala105 (CD3D).
分子量
Approximately 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa (CD3E) and 44-46 kDa (CD3D) based on Tris-Bis PAGE result.
純度
> 95% as determined by SDS-PAGE and HPLC.
活性
ELISA Data: Immobilized Cynomolgus CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Antibody, mFc Tag with EC50 of 13.9ng/ml determined by ELISA.
內毒素
< 1.0 EU per 1μg of the protein by the LAL method.
制劑
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
復溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
運輸與保存方法
冰袋運輸。-20℃至-80℃保存,一年有效期。
復溶后,-20 至 -80°C,在未開封狀態下保存 3-6 個月。復溶后,2-8°C 保存 2-7 天。
建議第一次使用時分裝凍存,避免反復凍融。
注意事項
1. 避免反復凍融。
2. 為了您的安全和健康,請穿實驗服并戴一次性手套操作。
3. 本產品僅作科研用途!
產品數據
HB220808
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